Ohmic heating-based extraction of biocompounds from cocoa bean shell

Cocoa bean shell (CBS), a by-product of the chocolate industry, was employed as substrate for sustainable recovery bioactive compounds using ohmic heating (OH). Total phenolic content and antioxidant activity treated CBS were optimized by experimental design. Maximum extraction (23 mg GAE/g CBS) obtained at 67 °C, 50 min 44% ethanol (v/v). The extracts under central point conditions 284.5 μM Fe2+/g extract (FRAP) 36.4 TE/g (DPPH). use OH increased when compared to conventional process (CH) (approximately 40%). An on chemical also observed, ranging from 4 20%. metabolic two methods (OH CH) evaluated in non-tumoral (HEK293T L929) tumoral cell lines (Caco-2, HT-29, HeLa). presented low toxicity cells ROS preventive effects. These characteristics make them ideal be used food processing formulation, well nutraceutical products due their protection. results an with higher environmental impact.